mouse monoclonal antibody against dnmt1  (Novus Biologicals)

Journal: Journal of Applied Oral Science

Article Title: DNA methyltransferases expressions in mice tongue exposed to waterpipe smoke

doi: 10.1590/1678-7765-2025-0665

Figure Lengend Snippet: Epithelial immunohistochemical expression of DNMT1 at each exposure time. Main images were magnified at 100x and zoomed sub-image at 400x. Epithelial cells are stained in the nuclei in brown according to exposure period.

Article Snippet: The sections were then incubated with mouse monoclonal antibody against DNMT1 (60B1220.1, 1:1500 dilution, Novus Biologicals, Centennial, EUA) and DNMT3b (NB300-516, 1:500 dilution, Novus Biologicals, Centennial, EUA) at 4°C overnight.

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Journal of Applied Oral Science

Article Title: DNA methyltransferases expressions in mice tongue exposed to waterpipe smoke

doi: 10.1590/1678-7765-2025-0665

Figure Lengend Snippet: Boxplots showing enzyme expression pattern according to exposure times (in days) on each tongue site for DNMT1 (a) and DNMT3b (b). The graphic presents the variable distribution of our data in a five number summary (minimum, first quartile, mean, upper quartile and maximum); circles=outliers; *=extreme outliers; †=statistically significant difference.

Article Snippet: The sections were then incubated with mouse monoclonal antibody against DNMT1 (60B1220.1, 1:1500 dilution, Novus Biologicals, Centennial, EUA) and DNMT3b (NB300-516, 1:500 dilution, Novus Biologicals, Centennial, EUA) at 4°C overnight.

Techniques: Expressing

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-DNMT1 , Novus Biologicals , Cat# NB100-56519; RRID: AB_2093819.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet: (A–D) To assess the binding of LRP1-ICD (A), and recruitment of epigenetic repressors DNMT1 (B), EZH2 (C), or MBD2 (D), to the promoter regions of DKK1 , SPARC , and TIMP2 in EME6/7t EECs treated with ASC-CM, ChIP-qPCR was used at the indicated time points (0, 6, 24, 48, 96, and 168 h). n = 3 technical replicates. Data are represented by mean ± SD. p values were determined using ANOVA test. (E) Bisulfite pyrosequencing analysis targeting the promoter regions of DKK1 , SPARC , and TIMP2 to assess CpG methylation levels in EME6/7t EECs exposed to ASC-CM (experimental duplicates: CM1 and CM2) or control (experimental duplicates: C01 and C02) for 21 days. The green box indicates CpG island, and the short, thick black line over the gene body represents the scale of 200 bp. The thicker, short blue box represents the probed sites. (F) Model for the underlying repressive actions by ASC-CM, which leads to the sequential recruitment of epigenetic repressors and subsequently marks the promoters of DKK1 , SPARC , and TIMP2 for methylation. LRP1-ICD initiates transcriptional repression of these genes. EZH2 and DNMT1 binding then enforces DNA methylation and establishes epigenetic silencing. See also – , and .

Article Snippet: Sonicated DNA fragments (~300–500 bp) were incubated with rabbit monoclonal anti-LRP1-ICD antibody (Abcam, Cat# ab92544), mouse monoclonal anti-YAP1 antibody (Novus Biologicals, Cat# NBP2–22117), rabbit monoclonal anti-Pan-TEAD antibody (Cell Signaling Technology, Cat# 13295S), mouse monoclonal anti-EZH2 antibody (Active Motif, Cat# 39076), goat polyclonal anti-MBD2 antibody (Novus Biologicals, Cat# NB100–55415), mouse monoclonal anti-DNMT1 antibody (Novus Biologicals, Cat# NB100–56519), normal goat IgG antibody (Abcam, Cat# ab37373), normal mouse IgG antibody (Millipore, Cat# NI03–100UG) or normal rabbit IgG antibody (Pierce Magnetic ChIP Kit, ThermoFisher Scientific, Cat# 26157) overnight at 4°C.

Techniques: Binding Assay, ChIP-qPCR, CpG Methylation Assay, Control, Methylation, DNA Methylation Assay

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Sonicated DNA fragments (~300–500 bp) were incubated with rabbit monoclonal anti-LRP1-ICD antibody (Abcam, Cat# ab92544), mouse monoclonal anti-YAP1 antibody (Novus Biologicals, Cat# NBP2–22117), rabbit monoclonal anti-Pan-TEAD antibody (Cell Signaling Technology, Cat# 13295S), mouse monoclonal anti-EZH2 antibody (Active Motif, Cat# 39076), goat polyclonal anti-MBD2 antibody (Novus Biologicals, Cat# NB100–55415), mouse monoclonal anti-DNMT1 antibody (Novus Biologicals, Cat# NB100–56519), normal goat IgG antibody (Abcam, Cat# ab37373), normal mouse IgG antibody (Millipore, Cat# NI03–100UG) or normal rabbit IgG antibody (Pierce Magnetic ChIP Kit, ThermoFisher Scientific, Cat# 26157) overnight at 4°C.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: eLife

Article Title: Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

doi: 10.7554/eLife.97507

Figure Lengend Snippet: ( A ) The expression levels of Dnmt1 , Dnmt3a, and Dnmt3l in oocytes were examined using qPCR after the treatment with SQ22536 and forskolin. *p<0.05. Data presented as mean ± SEM; a two-tail t-test was used. p-value presented in the source data. ( B ) The relative expressions of Dnmt1 , Dnmt3a, and Dnmt3l in oocytes were examined using qPCR after the treatment with luzindole and melatonin. *p<0.05. Data presented as mean ± SEM; a two-tail t-test was used. ( C ) After 8-Bromo-cAMP treatment, the relative expression of DNMT3a in oocytes was examined using immunofluorescence and calculated by Image J ( D ) (Control, n=54; 8-Bromo-cAMP, n=70, p=0.002447). **p<0.01. Data presented as mean ± SEM; a two-tail t-test was used. ( E and F ) Treatment with the protein kinase A (PKA) antagonist H 89 2HCL treatment significantly reduced the level of DNMT3A in oocytes examined using immunofluorescence (Control, n=62; H 89 2HCl, n=48, p=0.003922). **p<0.01. Data presented as mean ± SEM; a two-tail t-test was used. ( G and H ) DNMT1 localization in the oocyte nucleus was examined using immunofluorescence after 8-Bromo-cAMP treatment (Control, n=30; 8-Bromo-cAMP, n=31, p=3.136*10 –7 ). ***p<0.001. Data presented as mean ± SEM;a two-tail t-test was used. ( I and J ) The localization of DNMT1 in oocyte nucleus was reduced by the treatment with the PKA antagonist H 89 2HCL (Control, n=22; H 89 2HCl, n=28, p=0.004929). ** p<0.01. Data presented as mean ± SEM; a two-tail t-test was used. Source data are presented in . Figure 7—source data 1. Extended data for .

Article Snippet: Antibody , Anti-DNMT1 antibody (Mouse Monoclonal) , Active motif , 39204 , IF(1:200).

Techniques: Expressing, Immunofluorescence, Control

Journal: eLife

Article Title: Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

doi: 10.7554/eLife.97507

Figure Lengend Snippet: ( A ) The relative expression of Dnmt1 , Dnmt3a, and Dnmt3l in HFD oocytes was examined using qPCR. *p<0.05; **p<0.01. Data presented as mean ± SD; two-tail t-test used. p-value presented in the source data. ( B ) The concentration of cAMP in HFD oocytes was examined using ELISA. **p<0.01. Data presented as mean ± SEM; a two-tail t-test was used, n: CD=6, HFD = 3, p=0.004375.( C ) The relative expressions CREB1 and CREM in HFD oocytes were tested using qPCR. *p<0.05; **p<0.01. Data presented as mean ± SD; a two-tail t-test was used. Replicated three times for each gene, and p value presented in the source data.( D and E ) The level of pCREB1 in oocytes was examined using immunofluorescence, and the relative fluorescence intensity was calculated by Image J ( E ) (CD, n=69; HFD, n=49, p=3.326×10 –16 ; HFD + melatonin, n=61, p=8.997×10 –20 ). HFD, oocytes from obese mice; CD, oocytes from control mice; HFD + melatonin, oocytes from obese mice treated with exogenous melatonin. *p<0.05; ***p<0.001. Data presented as mean ± SEM; a two-tail t-test was used. ( F and G ) Treatment with the PKA antagonist H89 2HCL reduced the methylation level of HFD oocytes (CD, n=48; HFD, n=31, p=1.674*10 –13 ; HFD + H 89 2HCl, n=27, p=0.00324). ** p<0.01; *** p<0.001. Data presented as mean ± SEM; a two-tail t-test was used. ( H and I ) The level of pCREB1 in HFD oocytes was also decreased by the treatment with the protein kinase A (PKA) antagonist H89 2HCL (CD, n=17; HFD, n=17, p=0.006249; HFD +H 89 2HCl, n=22, p=0.027987). *p<0.05; **p<0.01; ns, no statistical significance between groups. Data presented as mean ± SEM; a two-tail t-test was used. ( J and K ) Treatment with the PKA antagonist H89 2HCL reduced the localization of DNMT1 in HFD oocytes (CD, n=24; HFD, n=29, p=6.214×10 –6 ; HFD + H 89 2HCl, n=25, p0.003147). **p<0.01; ***p<0.001; ns, no statistical significance between groups. Data presented as mean ± SEM; a two-tail t-test was used. Source data are presented in . Figure 8—source data 1. Extended data for .

Article Snippet: Antibody , Anti-DNMT1 antibody (Mouse Monoclonal) , Active motif , 39204 , IF(1:200).

Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence, Control, Methylation

Journal: eLife

Article Title: Maternal obesity may disrupt offspring metabolism by inducing oocyte genome hyper-methylation via increased DNMTs

doi: 10.7554/eLife.97507

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-DNMT1 antibody (Mouse Monoclonal) , Active motif , 39204 , IF(1:200).

Techniques: Sequencing, Enzyme-linked Immunosorbent Assay, Software

Journal: Frontiers in Immunology

Article Title: Amelioration effect of 18β-Glycyrrhetinic acid on methylation inhibitors in hepatocarcinogenesis -induced by diethylnitrosamine

doi: 10.3389/fimmu.2023.1206990

Figure Lengend Snippet: Measurement of the cofactor of methylation activity (DNMT1and MS) by qRT-PCR and flow cytometery. Positive control increased DNMT1 to 7-fold change while other treatment makes significant downregulation (A) . Error bars indicate standard deviation of three independent experiments. The student’s two-tailed t-test was used for statistical analysis. P-value ≤ 0.05 was considered statistically significant. Positive control increased MS to 10-fold change while other treatment makes significant downregulation (B) . Protein expression profile of DNMT1 and MS indicated by red and blue dotes using secondary antibodies Alex Flour-488 and 594, respectively (C) . *statistically significant difference as compared with the controls (P, 0.05 for each). While, ** statistically significant difference as compared with the controls (P, 0.01 for each).

Article Snippet: The same procedures were considered for staining DNMT1, MS, NFkB, and STAT3 using specific antibodies: mouse monoclonal anti-DNMT1 (Sigma-Aldrich, Germany), rabbit monoclonal anti-MS (Sigma-Aldrich, Germany), mouse monoclonal anti-NFkB (Sigma-Aldrich, Germany), and rabbit monoclonal anti-STAT3 (Sigma-Aldrich, Germany), respectively.

Techniques: Methylation, Activity Assay, Quantitative RT-PCR, Positive Control, Standard Deviation, Two Tailed Test, Expressing